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GB/T 40249-2021 English PDF

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GB/T 40249-2021: Code of diagnosis for infection with Penaeus monodon-type baculovirus - PCR method
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 40249-2021259 Add to Cart 3 days Code of diagnosis for infection with Penaeus monodon-type baculovirus - PCR method Valid

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Basic data

Standard ID: GB/T 40249-2021 (GB/T40249-2021)
Description (Translated English): Code of diagnosis for infection with Penaeus monodon-type baculovirus - PCR method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Word Count Estimation: 14,182
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 40249-2021: Code of diagnosis for infection with Penaeus monodon-type baculovirus - PCR method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Code of diagnosis for infection with Penaeus monodon-type baculovirus-PCR method ICS 65.020.30 CCSB41 National Standards of People's Republic of China Diagnosis of Penaeus monodon baculovirus disease PCR detection method PCRmethod Released on 2021-05-21 2021-12-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents. This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This document is under the jurisdiction of the National Fisheries Standardization Technical Committee (SAC/TC156). Drafting organizations of this document. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, National Fisheries Technology Extension Station. The main drafters of this document. Yang Bing, Wan Xiaoyuan, Huang Chi, Li Qing, Shi Chengyin, Zhang Feng, Song Xiaoling, Yu Weizhong, Zhang Qingli, Li Chen, Liu Li, Xu Hua. Diagnosis of Penaeus monodon baculovirus disease PCR detection method

1 Scope

This document specifies the requirements for nucleic acid detection technologies that are generally applicable in the diagnostic procedures for Penaeus monodon baculovirus disease. Penaeusmonodon-typebaculovirus (MBV), the reagents or materials, equipment, Sample, test procedure and result judgment. This document is applicable to the qualitative detection of MBV infection in samples of each life stage of shrimp, to carry out the flow of baculovirus disease of Penaeus monodon. Perform disease investigation, diagnosis, quarantine and monitoring.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to This document. GB/T 28630.4-2012 White Spot Syndrome (WSD) Diagnostic Procedure Part 4.Histopathological Diagnostic Method

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Abbreviations

The following abbreviations apply to this document. bp. base pair (basepair) DNA. deoxyribonucleic acid (deoxyribonucleicacid) dNTPs. deoxy-ribonucleosidetriphosphate EB. Ethidium bromide (ethidium bromide) EDTA. ethylenediaminetetraacetic acid (ethylenediaminetetraacetic acid) HCl. Hydrochloride (hydrogenchloride) MBV. Penaeusmonodon-typebaculovirus (Penaeusmonodon-typebaculovirus) NaOH. sodium hydroxide (sodiumhydroxide) PCR. polymerase chain reaction (polymerase chain reaction) SDS. Sodiumdodecylsulfate (sodiumdodecylsulfate) Taq. Thermus aquaticus (thermusaquaticus) TE. Tris hydrochloric acid and EDTA buffer (EDTATris·HCl) Tris. trishydroxymethylaminomethane (trishydroxymethylaminomethane)
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