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GB/T 40225-2021 English PDF

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GB/T 40225-2021: Determination of actin antibody - Western blotting method
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 40225-2021259 Add to Cart 3 days Determination of actin antibody - Western blotting method Valid

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Basic data

Standard ID: GB/T 40225-2021 (GB/T40225-2021)
Description (Translated English): Determination of actin antibody - Western blotting method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A40
Word Count Estimation: 14,171
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 40225-2021: Determination of actin antibody - Western blotting method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of actin antibody-Western blotting method ICS 07.080 CCSA40 National Standards of People's Republic of China Detection of Actin Antibody by Western Blot Released on 2021-05-21 2021-12-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents. This document was proposed and managed by the National Standardization Technical Committee for Biochemical Testing (SAC/TC387). Drafting organizations of this document. China Institute of Testing Technology, Chengdu Zhengneng Biotechnology Co., Ltd., Shenzhen Second People's Hospital, Gansu Zhongshang Food Quality Inspection and Testing Co., Ltd., Shenzhen Mindray Biomedical Electronics Co., Ltd., Shanghai Metrology and Testing Technology Research Institute Research Institute. The main drafters of this document. Li Huaiping, Zhou Lihua, Qiu Junkang, Ma Lixia, Gu Dayong, Zhang Qinchuan, Zhang Yiyi, Jiang Zijing, Wang Wei, Ye Deping, He Haining, Hong Xia, Liu Gang. Detection of Actin Antibody by Western Blot

1 Scope

This document describes the immunoblot detection method for actin antibodies. This document is suitable for the determination of actin antibodies based on the principle of western blotting.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to This document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Actin A type of globular multifunctional protein that forms microfilaments, the main component of the cytoskeleton, is widely expressed in eukaryotic cells. Note 1.At present, it has been found that there are at least 6 isoforms of actin, which are mainly distributed in the myocardium, skeletal striated muscle and smooth muscle tissues to regulate cell contraction Ability, also expressed in non-muscle cells, regulates cell structure and vitality. Note 2.The molecular weight of actin is 45ku±3ku (1u≈1Da), which is highly conservative and its expression in cells is relatively stable, so it is often used as Correct the internal parameters of the system. 3.2 Actin antibody Using actin as the antigen, the animal serum obtained after immunization of the animal can bind to actin-specific "antigen-antibody" immune The general term for phytoglobulin.

4 Abbreviations

The following abbreviations apply to this document. APS. ammonium peroxodisulphate BCA. bicinchoninic acid ECL. chemiluminescence reagent (electrogeneratedchemiluminescence) HRP. horseradish peroxidase (horseradishperoxidase) NP-40.ethyl phenyl polyethylene glycol (NonidetP40) PBS. phosphate buffer saline PBST. Phosphate Tween Buffer (phosphatebuffersalinewithTween) PMSF. phenylmethylsulfonylfluoride PVDF. polyvinylidenefluoride
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