GB/T 40138-2021 English PDF

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GB/T 40138-2021: Detection and identification of Southern bean mosaic virus
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GB/T 40138-2021	259	Add to Cart	3 days	Detection and identification of Southern bean mosaic virus	Valid

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Basic data

Standard ID: GB/T 40138-2021 (GB/T40138-2021)
Description (Translated English): Detection and identification of Southern bean mosaic virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 14,127
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 40138-2021: Detection and identification of Southern bean mosaic virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Southern bean mosaic virus ICS 65.020.01 CCSB16 National Standards of People's Republic of China Quarantine and identification method of southern bean mosaic virus Released on 2021-05-21 2021-12-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents. This document was proposed and managed by the National Plant Quarantine Standardization Technical Committee (SAC/TC271). This document was drafted by. Chinese Academy of Inspection and Quarantine Sciences, Guangzhou Customs Technology Center, Fruit Research Institute of Fujian Academy of Agricultural Sciences. The main drafters of this document. Zhang Yongjiang, Liu Han, Feng Lixia, Xie Lixue, Xiang Jun. Quarantine and identification method of southern bean mosaic virus

1 Scope

This document specifies the serological and molecular biological detection methods for southern bean mosaic virus. This document is applicable to the quarantine and identification of southern bean mosaic virus in legume plants and seeds.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to This document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Classification of Southern Bean Mosaic Virus

Chinese name. Southern Bean Mosaic Virus Scientific name. Southernbeanmosaicvirus Synonyms. Bean Mosaic Virus No. 4 Beanmosaicvirus 4; Southern Bean Mosaic Virus No. 1 Southernbeanmosaicvirus 1; Beansouthernmosaicvirus Taxonomic status. Southern bean mosaic virus order (Sobelivirales) Southern bean poinsettia mosaic virus family (Solemoviridae) South Sobemovirus See Appendix A for additional information on Southern Bean Mosaic Virus.

5 Principles of the method

The serological and genomic characteristics of southern bean mosaic virus are the basis for quarantine and identification of the virus. Southern Bean Mosaic Virus The specific reaction between the antibody and the double antibody sandwich enzyme-linked immunosorbent assay (DoubleAntibody Sandwich EnzymeLinkedImmunosorbentAssay, DAS-ELISA); to establish an inversion based on the genomic characteristics of southern bean mosaic virus Record recombinase polymerase amplification technology (ReverseTranscriptionRecombinasePolymeraseAmplification, RT-RPA) and Fluorescence RT-PCR detection method; through the effective combination of these methods, judge whether the sample contains Southern bean mosaic virus. 6 Reagents, equipment and utensils 6.1 Reagents Unless otherwise specified, all reagents are of analytical grade, and the experimental water should meet the relevant regulations in GB/T 6682. ......

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