SN/T 4684-2016 PDF English
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Determination of Burkholderia cepacia in cosmetics for import and export
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SN/T 4684-2016: Determination of Burkholderia cepacia in cosmetics for import and export---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/SNT4684-2016
SN
ENTRY AND EXIT INSPECTION AND QUARANTINE INDUSTRY
Determination of Burkholderia cepacia in cosmetics for
import and export
Issued on. DECEMBER 12, 2016
Implemented on. JULY 01, 2017
Issued by. General Administration of Quality Inspection and Quarantine of PRC
Table of Contents
Foreword... 3
1 Scope... 4
2 Normative references... 4
3 Equipment and materials... 4
4 Media and reagents... 5
5 Inspection procedures... 5
6 Operation procedures... 6
7 Report of results... 8
8 Waste disposal... 8
Appendix A (Normative) Media and reagents... 9
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some contents of this document may refer to patents. The issuing
agency of this document assumes no responsibility for identifying these patents.
This standard was proposed by AND shall be under the jurisdiction of the National
Certification and Accreditation Administration Committee.
Main drafting organizations of this standard. Shandong Entry-Exit Inspection and
Quarantine Bureau of the People's Republic of China, Perfect (China) Co., Ltd.
The main drafters of this standard. Zhao Han, Cui Shuhua, Sun Jun, Hao Ying, Tian
Guoning, Wu Xinghai, Gao Yecheng, Jia Juntao, Duan Xiaohui, Li Jianjun.
Determination of Burkholderia cepacia in cosmetics for
import and export
1 Scope
This standard specifies the detection method of Burkholderia cepacia in cosmetics for
import and export.
This standard applies to the qualitative detection of Burkholderia cepacia in cosmetics
for import and export.
2 Normative references
The following documents are essential to the application of this document. For the dated
documents, only the versions with the dates indicated are applicable to this document;
for the undated documents, only the latest version (including all the amendments) is
applicable to this standard.
GB 7918.1 Standard methods of microbiological examination for cosmetics -
General rules
3 Equipment and materials
In addition to the routine sterilization and culture equipment in the microbiology
laboratory, other equipment and materials are as follows.
3.1 Constant temperature incubator. 30 °C ± 1 °C, 32 °C ± 1 °C, 36 °C ± 1 °C.
3.7 Sterile petri dish. 90 mm in diameter (or other commercial disposable sterile petri
dish).
3.8 Inoculation loop. 3 mm diameter.
3.9 Homogenizing bag or homogenizing bottle.
3.10 Automatic microbial biochemical identification system.
4 Media and reagents
4.1 SCDLP enrichment solution containing polymyxin B. See A.1.
4.2 BCSA enrichment solution containing gentamicin. See A.2.
4.6 Reagent for oxidase test. See A.6.
4.7 Glucose oxidation/fermentation tube. See A.7.
4.8 Sucrose oxidation medium. See A.8.
4.9 Simon's citrate medium. See A.9.
4.10 Esculin medium. See A.10.
4.11 Biochemical identification kit.
5 Inspection procedures
See Figure 1 for the inspection procedure of Burkholderia cepacia.
6 Operation procedures
6.1 Enrichment culture
Prepare a 1.10 cosmetic dilution, according to the method of GB 7918.1.Take 10 mL
of dilution. Add it to 90 mL of SCDLP enrichment solution, which contains polymyxin
B. Incubate at 36 °C ± 1 °C for 24 h.
6.2 Isolation and culture
Take one ring of BCSA enrichment solution, by an inoculation loop. Mark a line, to
inoculate it on BCSA agar (containing polymyxin B, gentamicin) plate and MacConkey
agar (containing polymyxin B, gentamicin) plate. Culture it at 32 °C ± 1 °C for 48 h.
6.3 Identification
Sample inspection
6.3.1 Initial screening
Pick more than 5 typical or suspicious colonies from the selective agar plate. Inoculate
the same strain into two glucose oxidation/fermentation tubes, one of which is sealed
with sterilized liquid paraffin, whilst the other is not sealed. Culture it at 36 °C ± 1 °C
for 24 h.
6.3.5 Oxidation of sucrose
Pick suspicious colonies and inoculate them in sucrose oxidation medium. Incubate at
36 °C ± 1 °C for 24 h. If the culture turns yellow, it is positive; if it turns blue, it is
negative. Burkholderia cepacia is positive.
6.3.6 Utilization of citrate
Pick suspicious colonies and inoculate them in Simon's citrate medium. Incubate at
36 °C ± 1 °C for 24 hours. If the medium turns blue, it is positive; if it is light green, it
is negative. Burkholderia cepacia is positive.
6.3.7 Utilization of esculin
Pick suspicious colonies and inoculate them in esculin medium. Incubate at 30 °C ±
1 °C for 24 h. If the medium turns black, it is positive; if it does not change color, it is
negative. Burkholderia cepacia is positive.
6.3.8 Alternative tests
It can choose a biochemical identification kit or an automatic microbial biochemical
identification system, to identify suspicious colonies, according to the preliminary
judgment results in 6.3.1.
7 Report of results
Based on the above test results, it is reported that Burkholderia cepacia is detected or
not detected in 10 g (mL) of samples.
8 Waste disposal
The waste in the testing process shall be disposed of in accordance with the relevant
requirements of GB 19489.
Appendix A
(Normative)
Media and reagents
A.1 SCDLP enrichment solution containing polymyxin B
SN/T 4684-2016
SN
ENTRY AND EXIT INSPECTION AND QUARANTINE INDUSTRY
Determination of Burkholderia cepacia in cosmetics for
import and export
Issued on. DECEMBER 12, 2016
Implemented on. JULY 01, 2017
Issued by. General Administration of Quality Inspection and Quarantine of PRC
Table of Contents
Foreword... 3
1 Scope... 4
2 Normative references... 4
3 Equipment and materials... 4
4 Media and reagents... 5
5 Inspection procedures... 5
6 Operation procedures... 6
7 Report of results... 8
8 Waste disposal... 8
Appendix A (Normative) Media and reagents... 9
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some contents of this document may refer to patents. The issuing
agency of this document assumes no responsibility for identifying these patents.
This standard was proposed by AND shall be under the jurisdiction of the National
Certification and Accreditation Administration Committee.
Main drafting organizations of this standard. Shandong Entry-Exit Inspection and
Quarantine Bureau of the People's Republic of China, Perfect (China) Co., Ltd.
The main drafters of this standard. Zhao Han, Cui Shuhua, Sun Jun, Hao Ying, Tian
Guoning, Wu Xinghai, Gao Yecheng, Jia Juntao, Duan Xiaohui, Li Jianjun.
Determination of Burkholderia cepacia in cosmetics for
import and export
1 Scope
This standard specifies the detection method of Burkholderia cepacia in cosmetics for
import and export.
This standard applies to the qualitative detection of Burkholderia cepacia in cosmetics
for import and export.
2 Normative references
The following documents are essential to the application of this document. For the dated
documents, only the versions with the dates indicated are applicable to this document;
for the undated documents, only the latest version (including all the amendments) is
applicable to this standard.
GB 7918.1 Standard methods of microbiological examination for cosmetics -
General rules
3 Equipment and materials
In addition to the routine sterilization and culture equipment in the microbiology
laboratory, other equipment and materials are as follows.
3.1 Constant temperature incubator. 30 °C ± 1 °C, 32 °C ± 1 °C, 36 °C ± 1 °C.
3.7 Sterile petri dish. 90 mm in diameter (or other commercial disposable sterile petri
dish).
3.8 Inoculation loop. 3 mm diameter.
3.9 Homogenizing bag or homogenizing bottle.
3.10 Automatic microbial biochemical identification system.
4 Media and reagents
4.1 SCDLP enrichment solution containing polymyxin B. See A.1.
4.2 BCSA enrichment solution containing gentamicin. See A.2.
4.6 Reagent for oxidase test. See A.6.
4.7 Glucose oxidation/fermentation tube. See A.7.
4.8 Sucrose oxidation medium. See A.8.
4.9 Simon's citrate medium. See A.9.
4.10 Esculin medium. See A.10.
4.11 Biochemical identification kit.
5 Inspection procedures
See Figure 1 for the inspection procedure of Burkholderia cepacia.
6 Operation procedures
6.1 Enrichment culture
Prepare a 1.10 cosmetic dilution, according to the method of GB 7918.1.Take 10 mL
of dilution. Add it to 90 mL of SCDLP enrichment solution, which contains polymyxin
B. Incubate at 36 °C ± 1 °C for 24 h.
6.2 Isolation and culture
Take one ring of BCSA enrichment solution, by an inoculation loop. Mark a line, to
inoculate it on BCSA agar (containing polymyxin B, gentamicin) plate and MacConkey
agar (containing polymyxin B, gentamicin) plate. Culture it at 32 °C ± 1 °C for 48 h.
6.3 Identification
Sample inspection
6.3.1 Initial screening
Pick more than 5 typical or suspicious colonies from the selective agar plate. Inoculate
the same strain into two glucose oxidation/fermentation tubes, one of which is sealed
with sterilized liquid paraffin, whilst the other is not sealed. Culture it at 36 °C ± 1 °C
for 24 h.
6.3.5 Oxidation of sucrose
Pick suspicious colonies and inoculate them in sucrose oxidation medium. Incubate at
36 °C ± 1 °C for 24 h. If the culture turns yellow, it is positive; if it turns blue, it is
negative. Burkholderia cepacia is positive.
6.3.6 Utilization of citrate
Pick suspicious colonies and inoculate them in Simon's citrate medium. Incubate at
36 °C ± 1 °C for 24 hours. If the medium turns blue, it is positive; if it is light green, it
is negative. Burkholderia cepacia is positive.
6.3.7 Utilization of esculin
Pick suspicious colonies and inoculate them in esculin medium. Incubate at 30 °C ±
1 °C for 24 h. If the medium turns black, it is positive; if it does not change color, it is
negative. Burkholderia cepacia is positive.
6.3.8 Alternative tests
It can choose a biochemical identification kit or an automatic microbial biochemical
identification system, to identify suspicious colonies, according to the preliminary
judgment results in 6.3.1.
7 Report of results
Based on the above test results, it is reported that Burkholderia cepacia is detected or
not detected in 10 g (mL) of samples.
8 Waste disposal
The waste in the testing process shall be disposed of in accordance with the relevant
requirements of GB 19489.
Appendix A
(Normative)
Media and reagents
A.1 SCDLP enrichment solution containing polymyxin B
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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