PDF GB/T 31402-2023 English (GB/T 31402-2015: Older version)
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Measurement of antibacterial activity on plastics and other non-porous surfaces
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| GB/T 31402-2015 | English | 125 |
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Plastics -- Measurement of antibacterial activity on plastics surfaces
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GB/T 31402-2023: PDF in English (GBT 31402-2023) GB/T 31402-2023
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 83.080.01
CCS G 31
Replacing GB/T 31402-2015
Measurement of Antibacterial Activity on Plastics and Other
Non-Porous Surfaces
(ISO 22196.2011, IDT)
ISSUED ON. OCTOBER 27, 2023
IMPLEMENTED ON. JUNE 1, 2024
Issued by. State Administration for Market Regulation;
Standardization Administration of the People’s Republic of China.
Table of Contents
Foreword... 3
Introduction... 5
1 Scope... 6
2 Normative References... 7
3 Terms and Definitions... 7
4 Materials... 7
4.1 Bacteria to be used for the tests... 7
4.2 Reagents, culture media and solutions... 8
5 Apparatus... 10
6 Sterilization of Apparatus and Storage of Stock Cultures... 11
6.1 Dry-heat sterilization... 11
6.2 High-pressure steam sterilization... 12
6.3 Preparation of glassware... 12
6.4 Maintenance of stock cultures... 12
7 Procedure... 12
7.1 Pre-culture of bacteria... 12
7.2 Preparation of test specimens... 12
7.3 Preparation of test inoculum... 13
7.4 Inoculation of test specimens... 13
7.5 Incubation of the inoculated test specimens... 15
7.6 Recovery of bacteria from test specimens... 15
7.7 Determining the viable bacteria count by the pour plate culture method... 16
8 Expression of Results... 16
8.1 Determination of the number of viable bacteria... 16
8.2 Conditions for a valid test... 17
8.3 Calculation of the antibacterial activity... 17
8.4 Effectiveness of the antibacterial agent... 18
9 Repeatability and Reproducibility... 18
10 Test Report... 18
Annex A (Normative) Quality of Biological Materials... 20
Annex B (Informative) Repeatability and Reproducibility... 21
Annex NA (Informative) Calculation of Antibacterial Rate... 25
Bibliography... 26
Foreword
This Document was drafted as per the rules specified in GB/T 1.1-2020 Directives for
Standardization – Part 1.Rules for the Structure and Drafting of Standardizing Documents.
This Document replaced GB/T 31402-2015 Plastics - Measurement of Antibacterial Activity on
Plastics Surfaces. Compared with GB/T 31402-2015, the major technical changes of this
Document are as follows besides structural adjustments and editorial modifications.
a) Change the "Scope" to expand the scope of application from plastics to plastics and other
non-porous materials; and add descriptions of materials and products not covered by this
Document (see Clause 1 of this Edition; Clause 1 of the 2015 Edition);
b) Change the requirements for the use of "test bacteria" (see 4.1 of this Edition; 4.1 of the
2015 Edition);
c) Change the requirements for cleaning the specimen in "Preparation of specimens" (see
7.2 of this Edition; 7.2 of the 2015 Edition).
This Document equivalently adopted ISO 22196.2011 Measurement of Antibacterial Activity
on Plastics and Other Non-Porous Surfaces.
This Document made the following minimum editorial modifications.
--- Use Method B in the informative reference document GB/T 19275-2003 to replace
Method C in ISO 846.1997.The relevant technical contents of the two documents are
completely consistent (see Clause 1);
--- Domestic strain numbers equivalent to the tested strains are listed in Table 1 and
explained in footnotes;
--- Add Annex NA (informative) "Calculation of antibacterial rate".
Please note some contents of this Document may involve patents. The issuing agency of this
Document shall not identify these patents.
This Document was proposed by China Petroleum and Chemical Industry Federation.
This Document shall be under the jurisdiction of National Technical Committee on Plastics of
Standardization Administration of China (SAC/TC 15).
Drafting organizations of this Document. Institute of Microbiology, Guangdong Academy of
Sciences (Guangdong Detection Center of Microbiology); Guangdong Demay Biology
Technology Co., Ltd.; Guangzhou Research Institute of Synthetic Material Limited Company;
Jinda Nanotechnology (Xiamen) Co., Ltd.; Guangdong Huabiao Testing Center Co., Ltd.;
Chengdu Qinhuan Technology Co., Ltd.; Zhejiang Huiqian Nanotechnology Co., Ltd.; Cobes
Measurement of Antibacterial Activity on Plastics and Other
Non-Porous Surfaces
WARNING. Handling and manipulation of microorganisms which are potentially
hazardous requires a high degree of technical competence and may be subject to current
national legislation and regulations. Only personnel trained in microbiological techniques
should carry out such tests. Appropriate practices for disinfection, sterilization and
personal hygiene must be strictly observed.
1 Scope
This Document specifies a method of evaluating the antibacterial activity of antibacterial-
treated plastics, and other non-porous, surfaces of products (including intermediate products).
This Document is not intended to be used to evaluate the effects and propagation of bacteria on
non-porous surfaces without antibacterial treatments. If evaluation is required, see Method B
in GB/T 19275-2003[1].
Secondary effects of antibacterial treatments, such as the prevention of biodeterioration and
odor, are not covered by this Document, which is not intended to be used or referenced as a
method to document or claim biodegradability of, for instance, plastics materials. In the case of
plastics, biodegradation is covered in ISO 14851[2], ISO 14852[3] and ISO 14855[4] and related
standards.
Building materials are excluded, except where they are used in the same manner as treated
articles.
Antibacterial-treated textile products are excluded, even if the surfaces are coated or laminated
(such products are covered by ISO 20743[7]).
Photocatalytic materials and products are excluded (such materials and products are covered
by ISO 27447[8]).
The results obtained should include a reference to this Document and the conditions used.
Results obtained with this Document indicate antibacterial activity under the specified
experimental conditions used, and do not reflect activity under other circumstances where a
variety of factors, such as temperature, humidity, different bacterial species, nutrient conditions,
etc., have to be considered. A minimum diffusion of the antibacterial agents/chemicals into the
test inoculum is necessary with this procedure.
......
Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.
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