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GB/T 23873-2009: Determination of maduramicin ammonium in feeds
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Determination of maduramicin ammonium in feeds
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GB/T 23873-2009
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Basic data | Standard ID | GB/T 23873-2009 (GB/T23873-2009) | | Description (Translated English) | Determination of maduramicin ammonium in feeds | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 10,148 | | Date of Issue | 2009-05-26 | | Date of Implementation | 2009-10-01 | | Regulation (derived from) | PRC National Standard Approval Announcement 2009 No.7 (Total No.147) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the determination of maduramicin ammonium content in feed by high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS). This standard applies to feed, concentrated feed and feed additive premix. HPLC method for the quantitative determination. The limit of quantification is 1 mg/kg. LC-MS method for the qualitative determination methods. The lowest detection levels of 0. 5 mg/kg. | GB/T 23873-2009: Determination of maduramicin ammonium in feeds---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of maduramicin ammonium in feeds
ICS 65.120
B46
National Standards of People's Republic of China
Determination of maduramicin ammonium in feed
Posted 2009-05-26
2009-10-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
Appendix A of this standard is an informative annex.
This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) and focal points.
This standard was drafted. Ministry of Agriculture Feed Quality Supervision and Testing Center (Jinan).
The main drafters of this standard. Liang Meng, Tang Wenli, Renai Li, Zhao Jinyi Huang Ying LEE Wing Shan, Liu Xuejiang.
Determination of maduramicin ammonium in feed
1 Scope
This standard specifies the determination of maduramicin ammonium content in feed by high performance liquid chromatography (HPLC) and liquid chromatography - mass spectrometry
(LC-MS).
This standard applies to feed, concentrated feed and feed additive premix. HPLC method for the quantitative determination method, the limit of quantification
1mg/kg. LC-MS method for the qualitative determination of minimum detectable amount of 0.5mg/kg.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 14699.1 feed samples
GB/T 20195 Animal feed sample preparation
3 Sampling
3.1 sampling step. execution by GB/T 14699.1 requirements.
3.2 Preparation of samples. execution according to the provisions GB/T 20195 of.
4 HPLC method
4.1 Principle
Extraction feed maduramicin ammonium with acetonitrile, evaporated extract was dissolved with acetonitrile, with on - after dimethylaminobenzaldehyde solution was subjected to column Yan
Health, HPLC assay.
4.2 Reagents and materials
Unless otherwise specified, use only analytical reagent.
4.2.1 Water, GB/T 6682, level.
4.2.2 Methanol. chromatographically pure.
4.2.3 acetonitrile. chromatography.
4.2.4 tetrahydrofuran. chromatography.
4.2.5 potassium dihydrogen phosphate. pure class distinctions.
4.2.6 Phosphate. pure class distinctions.
4.2.7 - dimethylaminobenzaldehyde. 99%.
4.2.8 sulfuric acid. pure class distinctions.
4.2.9 Phosphate - potassium dihydrogen phosphate buffer solution (pH3.0). Weigh accurately 2.04g of potassium dihydrogen phosphate (4.2.5), add 900mL water dissolved,
Plus phosphate (4.2.6) several drops of pH was adjusted to 3.0 and transferred to 1000mL volumetric flask to volume with water, shake.
4.2.10 Mobile phase. phosphate - potassium dihydrogen phosphate buffer solution (4.2.9) + tetrahydrofuran (4.2.4) + methanol (4.2.2) = 100 + 40 + 860.
4.2.11 to - dimethylaminobenzaldehyde derivatized solution. Accurately weigh 16g of - dimethylaminobenzaldehyde (4.2.7) in 1000mL brown
Vial, was added 400mL of methanol (4.2.2), stirring until completely dissolved on a magnetic stirrer, the sulfuric acid was added dropwise 8mL
(4.2.8), and continue stirring until homogeneous solution was cooled to room temperature, provided with 24h.
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