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GA/T 1979-2022: Forensic sciences-Technical specifications for second generation sequencing for mitochondria) DNA
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Forensic sciences-Technical specifications for second generation sequencing for mitochondria) DNA
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GA/T 1979-2022
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Basic data | Standard ID | GA/T 1979-2022 (GA/T1979-2022) | | Description (Translated English) | Forensic sciences-Technical specifications for second generation sequencing for mitochondria) DNA | | Sector / Industry | Public Security (Police) Industry Standard (Recommended) | | Classification of Chinese Standard | A92 | | Classification of International Standard | 13.310 | | Word Count Estimation | 14,175 | | Date of Issue | 2022-03-09 | | Date of Implementation | 2022-08-01 | | Issuing agency(ies) | Ministry of Public Security | | Summary | This standard specifies the basic requirements for the terms and definitions, principles, testing equipment and reagents, testing procedures, and data analysis of human mitochondrial DNA next-generation sequencing testing conducted by forensic science DNA laboratories. This standard is applicable to forensic science DNA laboratories for the next-generation sequencing analysis of mitochondrial DNA for various forensic evidence materials and samples, and to provide mitochondrial DNA genetic information for forensic science practice. | GA/T 1979-2022: Forensic sciences-Technical specifications for second generation sequencing for mitochondria) DNA
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ICS 13.310
CCSA92
People's Republic of China Public Security Industry Standards
forensic science
Mitochondrial DNA Next Generation Sequencing Technical Specifications
Released on 2022-03-09
2022-08-01 Implementation
Released by the Ministry of Public Security of the People's Republic of China
foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules for Standardization Documents"
drafting.
Please note that some contents of this document may refer to patents. The issuing agency of this document assumes no responsibility for identifying patents.
This document was submitted by the Criminal Investigation Bureau of the Ministry of Public Security.
This document is under the jurisdiction of the Forensic Examination Sub-Technical Committee (SAC/TC179/SC6) of the National Criminal Technology Standardization Technical Committee.
This document was drafted by. Public Security Department of Liaoning Province, Dalian Public Security Bureau, Physical Evidence Appraisal Center of the Ministry of Public Security, Yantai Public Security Bureau, Guangzhou Public Security
Bureau, Weihai Public Security Bureau.
The main drafters of this document. Liu Feng, Li Mingguang, Zheng Zhejia, Ji Anan, Wang Le, Yu Jiao, Liu Li, Li Yue, Han Ying, Wang Weihua.
Forensic Science Mitochondrial DNA Next Generation Sequencing Specifications
1 Scope
This document specifies the terms and definitions, principles, and inspection methods for the next-generation sequencing of human mitochondrial DNA in forensic science DNA laboratories.
The basic requirements that should be complied with for test equipment and reagents, test procedures, data analysis, etc.
This document is suitable for forensic science DNA laboratories to conduct mitochondrial DNA next-generation sequencing for various forensic evidence materials and samples
Test analysis to provide mitochondrial DNA genetics information for forensic science practice.
2 Normative references
The contents of the following documents constitute the essential provisions of this document through normative references in the text. Among them, dated references
For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
this document.
GB/T 27025-2019 General requirements for the competence of testing and calibration laboratories
GB/T 30989-2014 Technical Regulations for High-throughput Gene Sequencing
GA/T 382-2014 Forensic Science DNA Laboratory Construction Specifications
GA/T 383-2014 Forensic Science DNA Laboratory Inspection Specification
GA/T 1161-2014 Content and format of forensic science DNA test identification documents
GA/T 1693-2020 Forensic Science DNA Next Generation Sequencing Inspection Specification
YY/T 1173-2010 Polymerase Chain Reaction Analyzer
3 Terms and Definitions
GB/T 30989-2014, GA/T 1693-2020 and the following terms and definitions apply to this document.
3.1
Mitochondrial DNA mitochondrialDNA; mtDNA
DNA outside the nucleus of human cells, which exists independently in the cytoplasm, has a closed-loop double-stranded structure, with about 16569 base pairs; does not follow Mendelian
The law of inheritance, manifested as maternal inheritance, the genetic information in the egg cell is passed on to the offspring; there is no cycle of mitosis and meiosis
The genetic material is located in the organelle and is not affected by nuclear transfer; the copy number of mtDNA in a single cell is large and there is heterogeneity.
3.2
heteroplasmy
When two or more mtDNA sequences exist in the same cell, tissue or individual, it is called heteroplasmy.
Note. It can be divided into length heterogeneity and point heterogeneity, which are common in the following two situations. (1) Different mtDNA sequences are detected in the same tissue in the same individual;
(2) Different mtDNA sequences were detected in different tissues of the same individual.
3.3
Hypervariable region hypervariableregion; HVR
In the DNA region located in the non-coding region of mtDNA, the mutation rate can reach 6-17 times that of the single-copy nuclear genome, and there is no repair system, no
Affected by selection pressure, more mutations were accumulated, and the polymorphism was good.
Note. Also known as the control region (control region), or D-loop region (D-loop region), usually divided into hypervariable region I according to the position on the mtDNA chain
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